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Blog: Biofilm Destruction
by Tizona

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Serrapeptase vs. Biofilm

does the enzyme serrateptase degrade biofilms? Yes it does!

Date:   12/3/2010 4:11:35 PM   ( 4 y ) ... viewed 5954 times

three excellent studies which demonstrate unequivocally that the enzyme serrapeptase (available to buy in supplement form) does in fact degrade biofilms.

One study shows not only does it degrade biofilms, but it also prevents their formation in the first place

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Listeria monocytogenes is a notably invasive bacterium associated with life-threatening food-borne disease in humans. Several surface proteins have been shown to be essential in the adhesion of L. monocytogenes, and in the subsequent invasion of phagocytes. Because the control of the invasion of host cells by Listeria could potentially hinder its spread in the infected host, we have examined the effects of a protease treatment on the ability of L. monocytogenes to form biofilms and to invade tissues. We have chosen serratiopeptidase (SPEP), an extracellular metalloprotease produced by Serratia marcescens that is already widely used as an anti-inflammatory agent, and has been shown to modulate adhesin expression and to induce antibiotic sensitivity in other bacteria. Treatment of L. monocytogenes with sublethal concentrations of SPEP reduced their ability to form biofilms and to invade host cells.


http://www.ncbi.nlm.nih.gov/pubmed/1847988...Pubmed_RVDocSum


Microbiological testing suggested that infection persisted in only one (5.6%) of eighteen animals in the serratiopeptidase-and-antibiotic group, whereas it was present in six (37.5%) of sixteen animals in the antibiotic-only group (p = 0.001). Histological evaluation showed similar results (kappa = 0.92). CONCLUSIONS: Serratiopeptidase was effective for eradicating infection caused by biofilm-forming bacteria in this experimental animal model. The antibiofilm property of the enzyme may enhance antibiotic efficacy in the treatment of staphylococcal infections.

http://www.ncbi.nlm.nih.gov/pubmed/1675775...Pubmed_RVDocSum


Among the different mechanisms of bacterial resistance to antimicrobial agents that have been studied, biofilm formation is one of the most widespread. This mechanism is frequently the cause of failure in the treatment of prosthetic device infections, and several attempts have been made to develop molecules and protocols that are able to inhibit biofilm-embedded bacteria. We present data suggesting the possibility that proteolytic enzymes could significantly enhance the activities of antibiotics against biofilms. Antibiotic susceptibility tests on both planktonic and sessile cultures, studies on the dynamics of colonization of 10 biofilm-forming isolates, and then bioluminescence and scanning electron microscopy under seven different experimental conditions showed that serratiopeptidase greatly enhances the activity of ofloxacin on sessile cultures and can inhibit biofilm formation.

http://www.ncbi.nlm.nih.gov/pubmed/8109925...Pubmed_RVDocSum

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Comments (9 of 9):
Re: cinnamon destr… Brigh… 8 mon
Re: cinnamon destr… Ladym… 14 mon
Re: Eucalyptus oil… #1527… 29 mon
Re: 15 essential o… patti… 31 mon
Re: Iodine degrade… Tizon… 4 y
Re: Iodine degrade… womba… 4 y
Re: Clove Oil vs. … womba… 4 y
Re: list of enzyme… Tizon… 4 y
Re: list of enzyme… #3878… 4 y
All Comments (9)

Blog Entries (6 of 12):
Serrapeptase vs. Biofilm  4 y
cinnamon destroys biofilms!  4 y
antibiotic proven to kill bi…  4 y
list of enzymes that may deg…  4 y
Amylase degrades biofilms?  4 y
vinegar destroys biofilms?  4 y
All Entries (12)

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