Vit. C Raises Glutathione by 50%
Vit. C Raises Glutathione by 50%
Date: 2/1/2009 12:18:37 PM ( 11 y ) ... viewed 3265 times
this study shows that a 500 mg. dose of Vitamin C boosts glutathione levels in red blood cells by 50%. Interestingly, a 2,000 mg dose didn't boost levels any more than a 500 mg dose of C. I wonder if adding some quercetin or polyphenols to the Vit C would help to boost glut levels even more?
Title: Vitamin C elevates red blood cell glutathione in healthy adults.
Author: Johnston, C S : Meyer, C G : Srilakshmi, J C
Citation: Am-J-Clin-Nutr. 1993 Jul; 58(1): 103-5
We examined the effect of supplemental ascorbic acid on red blood cell glutathione. Subjects consumed self-selected vitamin C-restricted diets, and, under double-blind conditions, ingested placebo daily for week 1 (baseline), 500 mg L-ascorbate/d for weeks 2-3, 2000 mg L-ascorbate/d for weeks 4-5, and placebo daily for week 6 (withdraw). Mean red blood cell glutathione rose nearly 50% (P less than 0.05) after the 500-mg period compared with baseline, and the changes from baseline for individual subjects ranged from +8% to +84%. However, the increases in plasma vitamin C and red blood cell glutathione were not correlated (r = 0.22). At the 2000-mg dosage, mean red blood cell glutathione was not significantly different from the value obtained at the 500-mg dosage. After the placebo-controlled withdraw period, red blood cell glutathione did not differ from baseline. These data indicate that vitamin C supplementation (500 mg/d) maintains reduced glutathione concentrations in blood and improves the overall antioxidant protection capacity of blood.
Vitamin C Protects Against Hypochlorous Acid–Induced Glutathione Depletion and DNA Base and Protein Damage in Human Vascular Smooth Muscle Cells
Andrew M. Jenner*; J. Emilio Ruiz; Christina Dunster; Barry Halliwell; Giovanni E. Mann; Richard C.M. Siow
From the Centre for Cardiovascular Biology and Medicine (A.J., E.R., C.D., G.E.M., R.C.M.S.), Guy’s, King’s and St. Thomas’ School of Biomedical Sciences, King’s College, University of London, London, UK, and the Department of Biochemistry (A.J., B.H.), National University of Singapore, Republic of Singapore.
Correspondence to Dr R.C.M. Siow, Centre for Cardiovascular Biology and Medicine GKT School of Biomedical Sciences, King’s College, University of London, Guy’s Hospital Campus, London SE1 1UL, UK. E-mail firstname.lastname@example.org
Hypochlorous acid (HOCl), generated by myeloperoxidase released from activated macrophages, is thought to contribute to vascular dysfunction and oxidation of low density lipoproteins (LDLs) in atherogenesis.
We have previously shown that HOCl exposure can cause chlorination and oxidation of isolated DNA and that vitamin C protects human arterial smooth muscle cells against oxidized LDL–mediated damage. We report in the present study that vitamin C attenuates HOCl-induced DNA base and protein damage and depletion of intracellular glutathione (GSH) and ATP in human arterial smooth muscle cells. Cells were pretreated in the absence or presence of 100 µmol/L vitamin C (24 hours) and then exposed to HOCl (0 to 500 µmol/L, 0 to 60 minutes) in the absence of vitamin C.
Intracellular GSH and ATP levels were depleted by HOCl treatment, and gas chromatography–mass spectroscopy revealed a concentration- and time-dependent increase in DNA base oxidation and protein damage (measured as 3-chlorotyrosine). Pretreatment of smooth muscle cells with vitamin C significantly reduced the extent of HOCl-induced DNA and protein damage and attenuated decreases in intracellular ATP and GSH. Our findings suggest that physiological levels of vitamin C provide an important antioxidant defense against HOCl-mediated injury in atherosclerosis.
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